基于线粒体细胞色素氧化酶b(cytb)全基因序列分析我国部分地区兔痒螨的遗传变异特征

doi:10.11843/j.issn.0366-6964.2017.04.015

Abstract

Abstract:

In order to investigate whether the genetic variation in Psoroptes cuniculi be affected by species of rabbit, temperature zone and geographical origin, the complete sequence of mitochondrial cytochrome b (cytb) gene of 68 P. cuniculi samples from 6 different provinces, including isolates from rex-rabbit (Shaanxi, Hebei, Henan and Hubei) and meat-type rabbit (Shandong and Sichuan), were amplified and sequenced for the first time. Then, we analyzed the genetic variability of those P. cuniculi isolates, inferred by cytb sequences. The full length of cytb gene from 68 P. cuniculi isolates were all 1 100 bp. These sequences contained 96 variable sites and 46 haplotypes. The values of haplotypes diversity (Hd=0.959 2) and nucleotide diversity (π=0.012 0) in overall China population indicated a high genetic diversity. The negative value of neutrality tests (Tajima's D=-0.444 01，Fu's Fs=-1.454 25)and the phenomenon of mismatch distribution curve with multiple peak suggested that P. cuniculi population in China occurred expansion event in the past. There was a certain genetic differentiation (Fst=0.262 2) and low gene flow (Nm=0.7) between different geographic populations. An analysis of molecular variance (AMOVA) showed that the genetic differentiation mainly occurred within the population. Haplotype network and NJ phylogenetic tree were both showed that P. cuniculi isolates from China did not cluster according to the species of rabbit, temperature zone and geographical origin. The results indicated that P. cuniculi population from 6 different provinces of China had a high level of genetic diversity and a certain genetic differentiation, however, the genetic structure based on the species of rabbit, temperature zone and geographical origin has not found.

CLC Number:

S852.746

ZHAO Xi-bin, WANG Bao-jian, JIAN Ke-ling, YANG Guang-you, LAI Wei-min, GU Xiao-bin. Analysis of the Characteristics of Genetic Variability within Psoroptes cuniculi Isolates in some Regions of China, Inferred by Mitochondrial Cytochrome Oxidase b Gene[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(4): 714-721.

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Analysis of the Characteristics of Genetic Variability within Psoroptes cuniculi Isolates in some Regions of China, Inferred by Mitochondrial Cytochrome Oxidase b Gene

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